“'Watching' Proteins in Fast Motion"
The structural integrity of proteins is maintained by many weak interactions; as a result, a folded protein may be subject to various local and global conformational fluctuations, and its thermodynamic stability may vary across regions or domains. In addition, a protein may be intrinsically disordered, sampling a dynamic conformational ensemble without forming well-defined and long-lasting tertiary contacts. More importantly, the requirement for function necessitates proteins to possess a certain degree of structural plasticity. Therefore, in order to provide a molecular level understanding of the protein structure-dynamics-function relationship, we need to obtain site-specific information on protein conformation, environment, dynamics, and/or stability. However, assessing any protein property in a site-specific manner is not easy since native spectroscopic signals often lack the needed specificity. In this talk, we will discuss our ongoing efforts to overcome this limitation. In particular, we will describe several examples, showing how standard biophysical techniques, such as circular dichroism (CD), infrared (IR), and fluorescence spectroscopies, can be used to reveal local electrostatic environment, solvent accessibility, conformation, and conformational stability of proteins.
The 2019 FAU Frontiers in Science lecture series runs from Friday, January 11 through Friday, April 5. Learn about topics such as the astrobiology of meteorites, to keeping your genome safe. Explore the sciences with eight researchers and innovators from across the globe and right here in our own community. We invited experts in the fields of Anthropology, Astronomy, Biology, Chemistry, Geosciences, Mathematics, and Physics. Science Dean, Dr. Ata Sarajedini states, “My hope is that these lectures stir you to deeper learning, explain your world, ignite your discussions, and bring the richness of the sciences to you.”
Find out more at: science.fau.edu/frontiers.php.
Friday, March 15, 2019 at 3:30pm to 6:00pm
Charles E. Schmidt College of Medicine, 126
777 Glades Road, Boca Raton, FL 33431